Differential diagnosis of hereditary hemolytic anemias in a single multiscreening test by TGA/chemometrics

摘要: A multi-screening test based on the coupling of thermogravimetry and chemometrics was optimized for differential diagnosis hereditary hemolytic anemias. The novel is able to simultaneously perform a simple fast sickle cell anemia, thalassemia, spherocytosis elliptocytosis in single analysis few microliters non-pretreated whole blood. thermogravimetric profile blood from patients affected by such disorders found be characteristic specific anemic status or disorder due membrane defects. In addition, chemometric tools were used validate model prediction process curves obtain 1 hour an accurate diagnosis. effectiveness evaluated comparing results with confirmatory analyses each disorder. TGA/chemometric made it possible first level congenital erythrocyte defects, including hemoglobinopathies defects same accuracy obtained molecular investigation. number Italian difficult cases, neonatal which conventional screening tests did not manage confirming high ability multiscreening test.