The Olfactory Receptor OR51E1 Is Present along the Gastrointestinal Tract of Pigs, Co-Localizes with Enteroendocrine Cells and Is Modulated by Intestinal Microbiota

摘要: The relevance of the butyrate-sensing olfactory receptor OR51E1 for gastrointestinal (GIT) functioning has not been considered so far. We investigated in young pigs distribution along GIT, its relation with some endocrine markers, variation age and after interventions affecting gut environment intestinal microbiota. Immuno-reactive cells chromogranin A (CgA) were counted cardial (CA), fundic (FU), pyloric (PL) duodenal (DU), jejunal (JE), ileal (IL), cecal (CE), colonic (CO) rectal (RE) mucosae. co-localization serotonin (5HT) peptide YY (PYY) evaluated PL CO respectively. FU tissues also sampled from 84 piglets reared sows receiving either or oral antibiotics (amoxicillin) around parturition, sacrificed at days 14, 21, 28 (weaning) 42 age. JE samples obtained 12 caesarean-derived that orally associated simple (SA) complex (CA) microbiota postnatal phase, which on 26–37 loops perfused 8 h enterotoxigenic Escherichia coli F4 (ETEC), Lactobacillus amylovorus saline (CTRL). Tissue densities OR51E1+ decreasing order: PL=DU>FU=CA>JE=IL=CE=CO=RE. showed an enteroendocrine nature containing hormones such as PYY 5HT. gene expression increased during suckling period (p<0.05). It was marginally reduced offspring antibiotic-treated (tendency, p=0.073), vs. control. Jejunal early SA, compared CA, ETEC-perfused CTRL (p<0.01). Our results indicate is related to GIT activity. Moreover age, pathogen challenge dietary manipulations influencing luminal microenvironment significantly affect presumably association release microbial metabolites.