The isolation and identification of a secreted biomarker associated with cell stress in serum-free CHO cell culture.

摘要: One approach to improving mammalian culture productivity has been reduce cell stress and death in the bioreactor, thus enhancing through a longer phase of viability. Here we describe isolation identification biomarker for viability loss CHO culture. Using SELDI-TOF mass spectrometry profile protein component supernatant media have identified peak at 7.7 kDa that was associated with toward end simulated from both toxic metabolite accumulation nutrient depletion. The relative intensity (signal/noise ratio) increased rapidly onset dropping growth phase. Also, height seen increase significantly when cells were grown under conditions emulating ammonia glutamine deprivation. species as fragment Galectin-1 (Gal-1) via MS/MS fingerprinting. We propose this could be utilized marker early This work demonstrates efficacy SELDI technology identify biomarkers highlights its value tool monitoring improvement processes.