Transcriptional regulation of calcium-activated neutral protease in cardiomyocytes of hypertensive rats
作者： M.N. Kumar , P. Grammas , F. Giacomelli , J. Wiener
DOI: 10.1016/1054-8807(92)90028-M
关键词: Northern blot 、 Regulation of gene expression 、 Messenger RNA 、 Genetic model 、 Endocrinology 、 Transcriptional regulation 、 Spontaneously hypertensive rat 、 Molecular biology 、 Kidney 、 Biology 、 Blot 、 Internal medicine
摘要: Increased enzymatic activity of calcium-activated neutral protease II (CANP II) has been reported previously in cardiac tissues rats with 2 kidney, 1 clip Goldblatt hypertension (2K, 1C-HT); this was associated elevated intracellular free Ca(++). Because it suggested that increased levels the enzyme were responsible for enhanced activity, CANP mRNA expression assessed cardiomyocytes isolated from 2K, 1C-HT and a genetic model hypertension, spontaneously hypertensive rat (SHR). Utilizing rabbit probe large subunit (pLM 1006 cDNA), 3.7 kilobase band visualized Northern blots poly A+ RNA. Densitometric analysis revealed there significant (p < 0.005) increase when compared controls. Interestingly, comparable SHR Wistar Kyoto (WKY) rats. Results nuclear runoff transcription assays indicated hearts regulated at transcriptional level. The data support specific gene activation renal
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