Loss of expression of the DCC gene during progression of colorectal carcinomas in familial adenomatous polyposis and non-familial adenomatous polyposis patients.
作者： Michiko Miyaki , Hiroyuki Fukunari , Rei Kikuchi-Yanoshita , Motoko Konishi , Kiyoko Tanaka
DOI:
关键词: Cancer research 、 Biology 、 Deleted in Colorectal Cancer 、 Colorectal cancer 、 Familial adenomatous polyposis 、 Tumor progression 、 Locus (genetics) 、 Cancer 、 Pathology 、 Loss of heterozygosity 、 Adenoma
摘要: Abstract We have previously observed that the frequency of loss heterozygosity (LOH) on chromosome 18q was low in adenomas and intramucosal carcinomas, whereas invasive carcinomas exhibited a high familial adenomatous polyposis patients (M. Miyaki et al. , Cancer Res., 50: 7166–7173, 1990). In present study, LOH at DCC locus expression gene into mRNA were analyzed colorectal tumors with distinct histopathological types. The showed also lost locus. examined level 233-base pair fragments nucleotide 986–1218 complementary DNA. moderate-to-severe adenoma, 5 carcinoma-in-adenomas, 4 as normal mucosa, it greatly reduced or not detectable most (13 16) carcinomas. Among these 7 11 LOH, but no LOH. These results suggest is included allelic deletion 18q, progression carcinoma from early stage to advanced accompanies inactivation through other mechanisms.
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