Wide-field photoacoustic microscopy of oxygen saturation at 1-MHz A-line rate

摘要: We report wide-field polygon-scanning optical-resolution photoacoustic microscopy (OR-PAM) that achieves 1-MHz A-line rate of oxygen saturation in vivo. develop a imaging probe. Using stimulated Raman scattering and optical delay fiber, we dual-wavelength pulsed laser has proper wavelengths, sufficient pulse energy, high repetition rate, sub-microseconds wavelength switching time for fast saturation. The OR-PAM system offers 478-Hz B-scan 1-Hz volumetric over 12×5 mm2 scanning area. demonstrate dynamic response to epinephrine injection the mouse ear. Results show hemodynamic functional changes centimeter-sized areas.