作者: T. Michael A. Wilson , Kitty A. Plaskitt , John W. Watts , Jane K. Osbourn , Peter A. C. Watkins
DOI: 10.1007/978-3-642-74164-7_7
关键词: Ribosome 、 Cowpea chlorotic mottle virus 、 Cell 、 RNA virus 、 RNA 、 Biology 、 Virus 、 Tobacco mosaic virus 、 Cell biology 、 Gene expression
摘要: One mechanism by which stable plant RNA virus particles might be disassembled in vivo involves recruitment of cell factors and ribosomes a short, easily exposed portion RNA. No specificity exists during uptake or early cotranslational disassembly. Virus-like reporter ribonucleocapsids were synthesized vitro to study the mechanism, sequence-dependence site disassembly transient gene expression without concomitant replication. “Pseudoviruses” transcapsidated viral RNAs can elucidate mechanism(s) genetically engineered, coat protein-mediated cross-protection electroporated tobacco protoplasts plants.