Altered microRNA Transcriptome in Cultured Human Liver Cells upon Infection with Ebola Virus.

摘要: Ebola virus (EBOV) is a virulent pathogen, notorious for inducing life-threatening hemorrhagic fever, that has been responsible several outbreaks in Africa and remains public health threat. Yet, its pathogenesis still not completely understood. Although there have numerous studies on host transcriptional response to EBOV, with an emphasis the clinical features, impact of EBOV infection post-transcriptional regulatory elements, such as microRNAs (miRNAs), largely unexplored. MiRNAs are involved inflammation immunity believed be important modulators viral infection. Here, we used small RNA sequencing (sRNA-Seq), qPCR functional analyses obtain first comparative miRNA transcriptome (miRNome) human liver cell line (Huh7) infected one following three strains: Mayinga (responsible Zaire outbreak 1976), Makona West 2013-2016) epizootic Reston (presumably innocuous humans). Our results highlight specific miRNA-based pathways substantial differences between strains beyond their manifestation pathogenicity. These shed new light into molecular signature cells upon reveal insights attack defense strategy.