Distribution and persistence of probiotic Streptococcus salivarius K12 in the human oral cavity as determined by real-time quantitative polymerase chain reaction.

摘要: The bacteriocin producer Streptococcus salivarius K12 is used as a probiotic targeting the oral cavity, so our study aimed to assess whether its dispersal and persistence could be monitored using real-time quantitative polymerase chain reaction. To this end, we designed reaction primers hybridization probe specifically salA, which encodes for prepropeptide of salivaricin A. Using single individual subject, administered four lozenges Throat Guard per day over 3 days, then measured salA gene levels 16 different sites at six intervals 35 days. Four samples each from gingival sulci teeth all remained negative. In contrast, in saliva mucosal membranes was detected, but with varying amounts time profiles. Relatively high copy numbers, calibrated on basis colony-forming units, were seen tongue (maximum 4.6 x 10(4)/swab 4), stimulated (2.4 10(4)/ml, 4) buccal (1.3 10(4)/swab, 8). present both sides pharynx asymmetrically quantity duration. conclusion, have developed quantitative-polymerase counting S. various cavity. studied, detected long weeks, steadily decreasing numbers after 8. Thus, may potential control bacterial infections only when uptake repeated frequently.