作者: J. F. Kirkpatrick , J. W. Turner , I. K. M. Liu
DOI:
关键词: Porcine zona pellucida 、 Sperm 、 Human fertilization 、 Biology 、 Luteinizing hormone 、 Sperm receptor 、 Andrology 、 Immunocontraception 、 In vitro fertilisation 、 Fertility
摘要: Reducing fertility among free-roaming feral horses (Equus caballus) has been the goal of numerous studies over past 16 years (Kirkpatrick et al. 1982, Goodloe 1988, Plotka 1989). Initial experiments by Kirkpatrick (1982) and Turner resulted in an 83% decrease foaling mares bred stallions which were first immobilized then treated with injectable microencapsulated testosterone propionate (mTP). Although treatment decreased sperm count motility, high costs stress caused immobilization or capture made it clear that contraceptive agent needed to be delivered remotely. In a second study (unpublished data) demonstrated pharmacological effectiveness mTP stallions, but difficulty was encountered remotely delivering sufficient mass steroid. Recently, attention turned contraception mare. Experiments ethinylestradiol-progesterone Silastic? implants (Vevea 1987, 1989) showed promise, technique required capture, restraint, field surgery place intraperitoneally. An alternative steroid-induced control is immunocontraception. A conjugated form luteinizing hormone releasing (LHRH) used successfully raise antibodies captive (Goodloe 1988), solubilized porcine zonae pellucidae (PZP) injections inhibited 13 14 domestic (Liu The success PZP vaccine suppressing based on its ability inhibit fertilization possibly implantation (Sacco 1984). zona pellucida consists 3 glycoproteins. One those, ZP3, receptor molecule for surface molecules (Florman Wassarman 1985). Equine raised against are thought block sites equine ovum, thereby preventing To date, number mammals, including 5 species nonhuman primates vitro humans 1987). objectives this (a) determine remote delivery, (b) test mares, (c) pregnant nonpregnant (d) evaluate safety use mares.