Evidence for Chelatable Zinc in the Extracellular Space of the Hippocampus, But Little Evidence for Synaptic Release of Zn

摘要: Zinc colocalizes with glutamate in the synaptic vesicles of certain glutamatergic mammalian brain. Here, I introduce a method for detecting Zn extracellular space brain slices and another passage out slice. In both cases, fluorimetric probe FluoZin-3 is used conjunction slow chelator, Ca-EDTA, to reduce background fluorescence. addition, new ethylenediiminodi-2-pentanedioic acid, little affinity Ca or Mg introduced. These tools are then show that (approximately 2 nm) released during course transmission into space. However, when hippocampal subjected high potassium stimulus (50 mM) combined an increase osmolarity, externalized Timm's-stained areas 6 nm). This also leads even greater elevations area CA1 only weakly stained by Timm's method. Nevertheless, under these conditions, if any makes its way slices. present evidence layer maps onto region hippocampus. veneer appears be loosely associated molecules may raison d'etre vesicular Zn.