Occurrence of Vibrio vulnificus in fish and shellfish available from markets in China.

作者: YUTAKA YANO , MASAHITO YOKOYAMA , MASATAKA SATOMI , HIROSHI OIKAWA , SHUN-SHENG CHEN

DOI: 10.4315/0362-028X-67.8.1617

关键词: KanamycinFood microbiologyMantis shrimpMicrobiologyPrawnVibrio vulnificusVeterinary medicineVibrionaceaeShellfishBiologyAmpicillin

摘要: Vibrio vulnificus is a naturally occurring estuarine bacterium often associated with disease such as septicemia in humans following consumption of raw and lightly cooked seafood. In China neighboring countries, rapid economic growth has encouraged increased seafood, dietary habits are changing, more people eating fish. this study, the prevalence V. was investigated 48 samples from 11 species live seafood available markets coastal cities China. The detected four razor clam samples, seven giant tiger prawn five nine mantis shrimp samples. also found water aquaria at markets. maximum level 3.4 log CFU/g 4.9 by direct spreading method. Differential bacterial counts on body revealed that most bacteria were shells (exoskeletons), very few edible muscle. However, dense populations can be intestines. Biochemical tests indicated isolates biotype 1 strain, which pathogenic to humans. These susceptible ampicillin, penicillin, kanamycin, streptomycin, erythromycin. results suggest potential health hazard consuming handling shellfish, especially prawns.

参考文章(35)
EIJI AONO, HARUO SUGITA, JUNJIRO KAWASAKI, HIROSHI SAKAKIBARA, TAKUMI TAKAHASHI, KAN ENDO, YOSHIAKI DEGUCHI, Evaluation of the polymerase chain reaction method for identification of Vibrio vulnificus isolated from marine environments. Journal of Food Protection. ,vol. 60, pp. 81- 83 ,(1997) , 10.4315/0362-028X-60.1.81
L A Brauns, M C Hudson, J D Oliver, Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells. Applied and Environmental Microbiology. ,vol. 57, pp. 2651- 2655 ,(1991) , 10.1128/AEM.57.9.2651-2655.1991
M L Tamplin, A L Martin, A D Ruple, D W Cook, C W Kaspar, Enzyme immunoassay for identification of Vibrio vulnificus in seawater, sediment, and oysters. Applied and Environmental Microbiology. ,vol. 57, pp. 1235- 1240 ,(1991) , 10.1128/AEM.57.4.1235-1240.1991
G Massad, J D Oliver, New selective and differential medium for Vibrio cholerae and Vibrio vulnificus. Applied and Environmental Microbiology. ,vol. 53, pp. 2262- 2264 ,(1987) , 10.1128/AEM.53.9.2262-2264.1987
DAVID W. COOK, PAUL O'LEARY, JEFF C. HUNSUCKER, EDNA M. SLOAN, JOHN C. BOWERS, ROBERT J. BLODGETT, ANGELO DePAOLA, Vibrio vulnificus and Vibrio parahaemolyticus in U.S. retail shell oysters: A national survey from June 1998 to July 1999 Journal of Food Protection. ,vol. 65, pp. 79- 87 ,(2002) , 10.4315/0362-028X-65.1.79
C A Kaysner, C Abeyta, M M Wekell, A DePaola, R F Stott, J M Leitch, Virulent strains of Vibrio vulnificus isolated from estuaries of the United States West Coast. Applied and Environmental Microbiology. ,vol. 53, pp. 1349- 1351 ,(1987) , 10.1128/AEM.53.6.1349-1351.1987
James D. Oliver, Robert A. Warner, David R. Cleland, Distribution of Vibrio vulnificus and Other Lactose-Fermenting Vibrios in the Marine Environment Applied and Environmental Microbiology. ,vol. 45, pp. 985- 998 ,(1983) , 10.1128/AEM.45.3.985-998.1983
K R O'Neill, S H Jones, D J Grimes, Seasonal incidence of Vibrio vulnificus in the Great Bay estuary of New Hampshire and Maine. Applied and Environmental Microbiology. ,vol. 58, pp. 3257- 3262 ,(1992) , 10.1128/AEM.58.10.3257-3262.1992
Tatsuo Kaneko, Rita R. Colwell, Adsorption of Vibrio parahaemolyticus onto Chitin and Copepods Applied Microbiology. ,vol. 29, pp. 269- 274 ,(1975) , 10.1128/AM.29.2.269-274.1975