Changes of androgen receptor and insulin-like growth factor-1 in LNCaP prostate cancer cells treated with sex hormones and flutamide.
作者： Da Yue Tong , Hong Yu Sun , Xin Yao Wu , Xing Qiao Wen , Zhuo Wei Liu
DOI:
关键词: Hormone 、 Prostate cancer 、 Androgen receptor 、 Blot 、 Endocrinology 、 Flutamide 、 LNCaP 、 Chemistry 、 Estrone 、 Internal medicine 、 Insulin-like growth factor
摘要: Changes of androgen receptor (AR) and insulin-like growth factor-1 (IGF-1) were investigated in LNCaP cells treated with 5α-dihydrotestosterone (DHT), estrone flutamide. Real-time PCR, immunocytochemistry western blotting used to detect the expression AR IGF-1 presence or absence various kinase inhibitors. Low concentrations DHT, flutamide increased IGF-1, especially estrone, concentration time dependence. With DHT flutamide, there was a significant alteration (p<0.001). The results indicated genes be influenced by cells, regulated multiple signal pathways.
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