Derivation of mesenchymal stem cells from human induced pluripotent stem cells cultured on synthetic substrates.

摘要: Human-induced pluripotent stem cells (hiPSCs) may represent an ideal cell source for research and applications in regenerative medicine. However, standard culture conditions that depend on the use of undefined substrates xenogeneic medium components a significant obstacle to clinical translation. Recently, we reported defined system human embryonic using synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), conjunction with xenogeneic-free medium. Here, tested hypothesis iPSCs could be maintained undifferentiated state this xeno-free subsequently differentiated into mesenchymal (iPS-MSCs). hiPSCs were cultured PMEDSAH functional MSCs, as confirmed by expression characteristic MSC markers (CD166+, CD105+, CD90+,CD73+, CD31-, CD34-, CD45-) their ability differentiate vitro adipogenic, chondrogenic, osteoblastic lineages. To demonstrate potential iPS-MSCs regenerate bone vivo, newly derived induced osteoblast differentiation 4 days transplanted calvaria defects immunocompromised mice 8 weeks. MicroCT histologic analyses demonstrated de novo formation animals treated but not control group. Moreover, positive staining nuclear antigen mitochondria monoclonal antibodies participation hiPS-MSCs regenerated bone. These results have capability MSCs form vivo.