ASN1-encoded asparagine synthetase in floral organs contributes to nitrogen filling in Arabidopsis seeds.

摘要: Despite a general view that asparagine synthetase generates as an amino acid for long-distance transport of nitrogen to sink organs, its role in metabolic pathways floral organs during seed filling has remained undefined. We demonstrate the onset pollination Arabidopsis induces selected genes metabolism, namely ASN1 (At3g47340), GLN2 (At5g35630), GLU1 (At5g04140), AapAT2 (At5g19950), ASPGA1 (At5g08100) and ASPGB1 (At3g16150), particularly at ovule stage (stage 0), accompanied by enhanced protein, total acids. Immunolocalization confined vascular cells silique cell wall septum, but also outer inner integuments, demonstrating post-phloem these developing embryos. In asn1 mutant, aberrant embryo divisions upper suspensor layers from globular heart stages assign differentiating embryos within ovary. Induction light/dark nitrate supports fine shifts pathways. transgenic expressing promoterCaMV35S ::ASN1 fusion, marked metabolomics changes 0, including several-fold increase free asparagine, are correlated nitrogen. However, specific promoterNapin2S expression formation six-fold toward desiccation result wild-type nitrogen, underlining delayed accumulation impairs timing use releasing amide Transcript metabolite profiles match carbon partitioning generate energy via tricarboxylic cycle, GABA shunt phosphorylated serine synthetic pathway.