Expression of human protection of telomere 1 correlates with telomere length and radiosensitivity in the human laryngeal cancer Hep-2 cell line.
作者： HAN LEI , DALI FENG , FUXIANG ZHOU , HUI XU , TIAN TANG
DOI: 10.3892/OL.2015.3332
关键词: Oncogene 、 Radioresistance 、 Apoptosis 、 Cell 、 Telomere 、 Cell culture 、 Cell cycle 、 Immunology 、 Biology 、 Radiosensitivity 、 Cancer research
摘要: The close association between telomere length and radiosensitivity has been established by several studies. There is also a hypothesis that may be regulated human protection of 1 (hPOT1) in carcinoma cells. In the present study, hPOT1 level radioresistant Hep-2R cells wild-type were compared, results showed gene was upregulated cell lines compared with wild-type. This suggested expression correlates radiosensitivity. Additionally, an hPOT1-directed short hairpin (sh)RNA plasmid constructed transferred into cells, which lead to shortening, increase apoptosis markedly decreased growth RNAi-Hep-2R line. These demonstrate shRNAs are associated radiosensitivity, maybe potent sensitizer for laryngeal cancer radiotherapy.
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