Comparative genetic and QTL mapping in sorghum and maize.

作者: M Lee

DOI:

关键词: Plant breedingGeneticsPlant geneticsBiologyGenetic diversityQuantitative trait locusGene mappingPopulationGenetic markerRestriction fragment length polymorphism

摘要: DNA markers and genetic maps will be important tools for direct investigations of several facets crop improvement provide vital links between plant breeding basic biology. The become more increased production because genetics required to extend or replace extant management practices such as chemical fertilizers, pesticides, irrigation (Lee, 1995). Despite the importance sorghum crop, comprehensive characterization has been limited. Therefore, primary goal this research program was develop facilitate in sorghum. first phase project consisted constructing a map based on restriction fragment length polymorphisms (RFLPs). ISU created through linkage analysis 78 F2 plants an intraspecific cross inbred CK60 accession P1229828 (Pereira et al., 1994). consists 201 loci distributed among 10 groups covering 1,299 cM. Comparison maize RFLP basis common sets probes revealed high degree conservation reflected by homology, copy number, collinearity. Examples conserved rearranged locus orders were observed. same population used factors (mutants QTL) traits including vegetative reproductive morphology, maturity, insect, disease resistance. This presentation emphasize affecting height, character adaptation temperate latitudes grain production. Four QTL height identified sample 152 Lee, 1995) whereas 6 detected their F3 progeny. These observations assessments other at 4 progeny indicate some these regions correspond (dw) previously alleles with highly qualitative effects. six seem orthologous maize. Other possible instances included maturity tillering. suggest that genomes encompasses sequence collinearity, function. information technology developed could breeding, genetics, biological investigations. In addition, have survey large collections elite germ plasm determine relationships diversity (Ahnert 1996). data portray accurately than methods exclusively coancestry coefficient. provides accurate perceptions diversity.

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