PARAMETERS AFFECTING FUSION BETWEEN SENDAI VIRUS AND LIPOSOMES - ROLE OF VIRAL-PROTEINS, LIPOSOME COMPOSITION, AND PH

摘要: A kinetic and quantitative characterization of the fusion process between Sendai virus phospholipid vesicles is presented. Membrane was monitored in a direct continuous manner by employing an assay which relies on relief fluorescence self-quenching probe octadecylrhodamine B chloride located viral membrane. Viral activity strongly dependent vesicle lipid composition most efficient with solely consisting acidic phospholipids, particularly cardiolipin (CL). This result implies that viruses liposomes does not display absolute requirement for specific membrane receptors. Incorporation phosphatidylcholine (PC), rather than phosphatidylethanolamine (PE), into CL bilayers inhibited fusion, suggesting repulsive hydration forces interfere close approach target Virus-liposome products were capable fusing liposomes, but virus. In contrast to erythrocyte membranes, triggered immediately, did strictly depend protein conformation, pH optimum around 7.5. On other hand, PC, PE, cholesterol, ganglioside GD1a, resembled more closely fusogenic properties seen membranes. Upon decreasing below 5.0, increased dramatically. With vesicles, maximal observed 4.0, while GD1a-containing zwitterionic continued increase down values as low 3.0.(ABSTRACT TRUNCATED AT 250 WORDS)