Obese gene expression: reduction by fasting and stimulation by insulin and glucose in lean mice, and persistent elevation in acquired (diet-induced) and genetic (yellow agouti) obesity.

摘要: Abstract Mutations in the obese (ob) gene lead to obesity. This has been recently cloned, but factors regulating its expression have not elucidated. To address regulation of ob with regard body weight and nutritional factors, Northern blot analysis was used assess mRNA adipose tissue from mice [lean, due diet, or genetically (yellow agouti) obese] under different conditions. elevated both forms obesity, compared lean controls, correlated elevations plasma insulin weight, glucose. In C57BL/6J mice, diet-induced decreased after a 48-hr fast. Similarly, yellow i.p. glucose injection significantly increased mRNA. For up 30 min injection, glucose, insulin. separate study only inhibited >90% by fasting, approximately 2-fold either These results suggest that animals enhance expression. contrast our is relatively insensitive state, possibly chronic exposure and/or