Osmotic stress as a factor in the detrimental effect of glycerol on human platelets.

摘要: Abstract The aim of this work was to determine the importance osmotic stress as a damaging factor in detrimental effect glycerol on human platelets. severity mitigated by reducing rate change concentration suspending medium. classical permeability equations were used predict cell volume changes response step extracellular concentration. Protocols devised that limited cellular shrinkage during addition and swelling dilution. When added diluted rapidly, recovery hypotonie with respect untreated controls unaffected 0.25 mol/liter glycerol, but reduced ca. 65% after exposure 0.5 25% 1 glycerol. removed slowly such remained within range 60–130% normal volume, hypotonic improved 50%, aggregation undiminished. Osmotic therefore at least partly responsible for damage caused However, platelets damaged more slow dilution from when 116% than rapid or which resulted 123% 146% respectively. Thus, possible toxic cannot yet be discounted.