Selection, Characterization, and Biosensing Application of High Affinity Congener-Specific Microcystin-Targeting Aptamers
作者： Andy Ng , Raja Chinnappan , Shimaa Eissa , Hechun Liu , Chaker Tlili
DOI: 10.1021/ES301686K
关键词: Nonspecific binding 、 DNA Aptamers 、 Microcystin 、 Congener specific 、 Aptamer 、 Biosensor 、 Chromatography 、 Chemistry 、 Detection limit
摘要: The efficiency of current microcystin detection methods has been hampered by the low detection limits required in drinking water and that routine detection is restricted to a few of the congeners with high degree of undesired cross-reactivity. Here, we report the development of novel microcystin-targeting molecules and their application in microcystin detection. We have selected DNA aptamers from a diverse random library that exhibit high affinity and specificity to microcystin-LR,-YR, and-LA. We obtained aptamers that bind to all …
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