Assembly of the Reovirus Genome

摘要: During the early days of molecular virology, presumed monomolecular nature their genomes was considered to be one primary virtues viruses. However, in 1969, irrefutable evidence surfaced that reovirus consist not one, but ten molecules double-stranded (ds)RNA (Shatkin et al. 1968). Further, it quickly became apparent this a case “headful” mechanism at work, such as appears operate for influenza virus, where virus particles probably contain random 11-segment collections eight actual genome segment species, so roughly 25 contains least each and is therefore infectious (Lamb Choppin 1983; Enami 1991). By contrast, assembly segments form an extraordinarily efficient precise process because ratio units carefully handled preparations essentially 1 (1.6 or less) (Spendlove 1970; Larson 1994).