Silencing of MusaANR1 gene reduces proanthocyanidin content in transgenic banana plants

作者: Siddhesh B Ghag , Upendra KS Shekhawat , Thumballi R Ganapathi , None

DOI: 10.1007/S11240-015-0739-X

关键词: BiologyAnthocyanidin reductaseBotanyPhenylpropanoidAnthocyaninPlant physiologyTransgeneFlavonoid biosynthesisSmall hairpin RNAProanthocyanidin

摘要: Manipulating flavonoid biosynthesis by genetic engineering in economically important crop plants is an emerging area of importance plant biotechnology. Banana, which the world most fruit production volumes, rich proanthocyanidins. We hypothesized that downregulating critical proanthocyanidin synthesis branch point enzyme, namely anthocyanidin reductase (MusaANR1) using intron-mediated hairpin RNA against its transcript, levels end product other phenylpropanoid pathway could be enhanced. Although, transcript MusaANR1 were successfully downregulated transgenic banana plants, there was no significant elevation anthocyanin tissues.

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