Low levels of interferon-alpha induce CD86 (B7.2) expression and accelerates dendritic cell maturation from human peripheral blood mononuclear cells.

作者: Radvanyi , Banerjee , Weir , Messner

DOI: 10.1046/J.1365-3083.1999.00625.X

关键词: Tumor necrosis factor alphaCD86MacrophageDendritic cellBiologyImmunologyColony-stimulating factorPeripheral blood mononuclear cellMolecular biologyMonocyteCytokine

摘要: Interferon-alpha (IFN-alpha) (IFN-alpha2b) is an immunoregulatory cytokine that presently used in a recombinant form for the treatment of tumours and chronic viral infection. However, its mechanism action remains largely undefined. In this paper, we studied effects low doses IFN-alpha (0-100 U/ml) on generation dendritic cells with granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-4 (IL-4), tumour necrosis (TNF)-alpha cultures human peripheral blood mononuclear (PBMCs). An addition to PBMC greatly increased HLA class II CD86 expression developing (DCs) during 7-day culture period. When added at initiation culture, as little 10 U/ml dramatically expression, maximal observed between 50 100 all preparations tested. Almost nonadherent induced possessed phenotype mature DCs, being CD1a(low), CD83+, IIhigh, CD86high, CD40high, CD80low, while negative monocyte/macrophage lymphocyte markers. contrast, floating isolated from grown without were mostly immature DCs CD1a(high), CD83-, IIint/high, CD86low/int, CD80low phenotype. time both number generated their rate appearance; by 3 days many large aggregates present containing CD1a(low) much fewer found IFN-alpha. Histochemical staining confirmed had typical DC features, including irregularly shaped nuclei, few cytoplasmic granules, absent or diffuse perinuclear esterase. Our results suggest potent accelerator maturation vitro. These may explain clinical success cancer infection well ability promote autoimmunity.

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