Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2.
作者： Claudia Solari , Camila Vázquez Echegaray , Carlos Luzzani , María Soledad Cosentino , Ariel Waisman
DOI: 10.1016/J.BBRC.2016.03.077
关键词: Induced stem cells 、 Molecular biology 、 Cellular differentiation 、 Rex1 、 Embryonic stem cell 、 Induced pluripotent stem cell 、 Homeobox protein NANOG 、 SOX2 、 Protein arginine methyltransferase 8 、 Biology
摘要: Addition of methyl groups to arginine residues is catalyzed by a group enzymes called Protein Arginine Methyltransferases (Prmt). Although Prmt1 essential in development, its paralogue Prmt8 has been poorly studied. This gene was reported be expressed nervous system and involved neurogenesis. In this work, we found that mouse embryonic stem cells (ESC) induced pluripotent cells, modulated along differentiation neural precursor cells. We promoter activity the pluripotency transcription factors Oct4, Sox2 Nanog. Moreover, endogenous mRNA levels were reduced ESC transfected with shRNA vector. As whole, our results indicate factors. These findings suggest besides known function system, could play role
sci-hub.se 参考文章(32)
Zoltan Simandi, Erik Czipa, Attila Horvath, Aron Koszeghy, Csilla Bordas, Szilárd Póliska, István Juhász, László Imre, Gábor Szabó, Balazs Dezso, Endre Barta, Sascha Sauer, Katalin Karolyi, Ilona Kovacs, Gábor Hutóczki, László Bognár, Álmos Klekner, Peter Szucs, Bálint L Bálint, Laszlo Nagy, None, PRMT1 and PRMT8 Regulate Retinoic Acid‐Dependent Neuronal Differentiation with Implications to Neuropathology Stem Cells. ,vol. 33, pp. 726- 741 ,(2015) , 10.1002/STEM.1894
J. Najbauer, B.A. Johnson, A.L. Young, D.W. Aswad, Peptides with sequences similar to glycine, arginine-rich motifs in proteins interacting with RNA are efficiently recognized by methyltransferase(s) modifying arginine in numerous proteins. Journal of Biological Chemistry. ,vol. 268, pp. 10501- 10509 ,(1993) , 10.1016/S0021-9258(18)82227-X
Jonghwan Kim, Jianlin Chu, Xiaohua Shen, Jianlong Wang, Stuart H. Orkin, An extended transcriptional network for pluripotency of embryonic stem cells Cell. ,vol. 132, pp. 1049- 1061 ,(2008) , 10.1016/J.CELL.2008.02.039
Myles B. C. Dillon, Heather L. Rust, Paul R. Thompson, Kerri A. Mowen, Automethylation of Protein Arginine Methyltransferase 8 (PRMT8) Regulates Activity by ImpedingS-Adenosylmethionine Sensitivity Journal of Biological Chemistry. ,vol. 288, pp. 27872- 27880 ,(2013) , 10.1074/JBC.M113.491092
Ivan Dikic, Ming-Ming Zhou, Tony Pawson, Bruce T. Seet, Reading protein modifications with interaction domains Nature Reviews Molecular Cell Biology. ,vol. 7, pp. 473- 483 ,(2006) , 10.1038/NRM1960
Carlos Luzzani, Claudia Solari, Noelia Losino, Waisman Ariel, Leonardo Romorini, Carolina Bluguermann, Gustavo Sevlever, Lino Barañao, Santiago Miriuka, Alejandra Guberman, Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells Biochemical and Biophysical Research Communications. ,vol. 410, pp. 816- 822 ,(2011) , 10.1016/J.BBRC.2011.06.070
Jaeho Lee, Joyce Sayegh, Jeremy Daniel, Steven Clarke, Mark T. Bedford, PRMT8, a New Membrane-bound Tissue-specific Member of the Protein Arginine Methyltransferase Family Journal of Biological Chemistry. ,vol. 280, pp. 32890- 32896 ,(2005) , 10.1074/JBC.M506944200
A. Kousaka, Y. Mori, Y. Koyama, T. Taneda, S. Miyata, M. Tohyama, The distribution and characterization of endogenous protein arginine N-methyltransferase 8 in mouse CNS Neuroscience. ,vol. 163, pp. 1146- 1157 ,(2009) , 10.1016/J.NEUROSCIENCE.2009.06.061
Joyce Sayegh, Kristofor Webb, Donghang Cheng, Mark T. Bedford, Steven G. Clarke, Regulation of protein arginine methyltransferase 8 (PRMT8) activity by its N-terminal domain. Journal of Biological Chemistry. ,vol. 282, pp. 36444- 36453 ,(2007) , 10.1074/JBC.M704650200
Keisuke Okita, Tomoko Ichisaka, Shinya Yamanaka, Generation of germline-competent induced pluripotent stem cells Nature. ,vol. 448, pp. 313- 317 ,(2007) , 10.1038/NATURE05934