Production of pyruvate from (R)-lactate in an enzyme–membrane reactor with coupled electrochemical regeneration of the artificial mediator anthraquinone-2,6-disulfonate

摘要: Abstract The production of pyruvate from ( R )-lactate using the enzyme (2 )-hydroxycarboxylate-viologen-oxidoreductase in form a crude extract Proteus vulgaris cells was studied an enzyme–membrane reactor with coupled electrochemical regeneration mediator. artificial mediator anthraquinone-2,6-disulfonate served as electron acceptor. A cross-flow membrane module used for retention. kinetics cell and enzymatic reaction rates were measured simple mechanistic modeling. Model calculations agreed well experimental data. overall performance limited by fouling; however, optimized operating conditions, deactivation practically eliminated. high activity could be maintained reactor; thus, rendering possible future preparative scale this synthesis dehydrogenation numerous other )-hydroxycarboxylates.