DcSto: carrot Stowaway-like elements are abundant, diverse, and polymorphic.

作者: Alicja Macko-Podgorni , Anna Nowicka , Ewa Grzebelus , Philipp W. Simon , Dariusz Grzebelus

DOI: 10.1007/S10709-013-9725-6

关键词: Consensus sequenceSequence alignmentEuchromatinGene dosageGeneGenomeGeneticsBiologyTransposable elementPromoter

摘要: We investigated nine families of Stowaway-like miniature inverted-repeat transposable elements (MITEs) in the carrot genome, named DcSto1 to DcSto9. All them were AT-rich and shared a highly conserved 6 bp-long TIR typical for Stowaways. The copy number was estimated as ca. 5,000 per diploid genome. observed preference clustered insertions DcSto other MITEs. Distribution hybridization signals revealed presence clusters within euchromatic regions along all chromosomes. An arrangement eight encompassing insertion sites, studied detail, variable among plants representing different populations Daucus carota. these polymorphic which most likely suggests very recent mobilization those elements. Insertions near genes putative promoters, regulatory motifs, polyA their sequences might suggest possible involvement regulation gene expression.

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