Application of genome-editing systems to enhance available pig resources for agriculture and biomedicine.

摘要: Traditionally, genetic engineering in the pig was a challenging task. Genetic of somatic cells followed by cell nuclear transfer (SCNT) could produce genetically engineered (GE) pigs carrying site-specific modifications. However, due to difficulties genome and developmental defects associated with SCNT, limited number GE models were reported. Recent developments genome-editing tools, such as zinc finger nucleases (ZFNs), transcription activator-like effector (TALENs) clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) 9 system, have markedly changed effort time required models. The frequency is now practical. In addition, SCNT no longer essential producing modifications, because direct injection systems into developing embryos introduces targeted To date, CRISPR/Cas9 system most convenient, cost-effective, timely commonly used technology. Several applicable biomedical agricultural been generated using system. Although efficiency has enhanced use systems, improvements are still needed optimally emerging Current future advances strategies will monumental effect on agriculture biomedicine.