MiR-193b-5p protects BRL-3A cells from acrylamide-induced cell cycle arrest by targeting FoxO3
作者： Yuan Yuan , Bo Nan , Haiyang Yan , Haiqing Ye , Minghua Wang
DOI: 10.1016/J.FCT.2021.112059
关键词: Ectopic expression 、 Cell cycle 、 Gene knockdown 、 Cell biology 、 Kinase 、 microRNA 、 Cell growth 、 Chemistry 、 Cyclin D1 、 Cell cycle checkpoint
摘要: Acrylamide (AA), an important by-product of the Maillard reaction, has been reported to be genotoxic and carcinogenic. The present study employed miRNAs investigate toxic mechanism AA their role against toxicity. Deep sequencing small RNA libraries was performed miR-193b-5p applied for further study. significantly reduced level its ectopic expression promoted cell cycle G1/S transition proliferation by upregulating cyclin-dependent kinase regulator Cyclin D1 downregulating inhibitor p21, while led opposite results. Dual luciferase assay demonstrated regulated FoxO3 directly targeting 3'-untranslated region (3'-UTR). Knockdown induced proliferation, which suppressed inhibition but mimics. MiR-193b-5p strengthened effect FoxO3, contrary In conclusion, acted as a mediate p21 D1.
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