Evaluation of genetic variation in Lachenalia bulbifera (Hyacinthaceae) using RAPDs.
作者： Riana Kleynhans , Johan J. Spies
关键词: Genetic variability 、 Botany 、 Lachenalia bulbifera 、 Biology 、 RAPD 、 Dendrogram 、 Genetic distance 、 Lachenalia 、 Genetic marker 、 Genetic variation
摘要: RAPD (randomly amplified polymorphic DNA) analyses were carried out on 21 accessions of Lachenalia bulbifera (Cyrillo) Engl. Five pre-selected primers produced an average 88% polymorphisms. Fifteen the could be identified using five primers. In a pairwise comparison genetic distance values ranging from 0.11 to 1.08 obtained. These reveal high amount variation within species. The tetraploid and hexaploid groups south coast low, but between those west coast. A dendogram was constructed banding profiles, UPGM cluster analysis. clusters certain together. are supported by their geographical locality chromosome data. group, group octoploid respectively clustered It is concluded that RAPDs can used assess at intra-specific level in Lachenalia.
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sci-hub.se 参考文章(34)
C. J. Liu, Genetic diversity and relationships among Lablab purpureus genotypes evaluated using RAPD as markers Euphytica. ,vol. 90, pp. 115- 119 ,(1996) , 10.1007/BF00025167
Diego Fajardo, Fernando Angel, Mikkel Grum, Joe Tohme, Mario Lobo, William M. Roca, Inés Sanchez, Genetic variation analysis of the genus Passiflora L. using RAPD markers Euphytica. ,vol. 101, pp. 341- 347 ,(1998) , 10.1023/A:1018395229851
P. Obara-Okeyo, S. Kako, Genetic diversity and identification of cymbidium cultivars as measured by random amplified polymorphic DNA (RAPD) markers Euphytica. ,vol. 99, pp. 95- 101 ,(1998) , 10.1023/A:1018374226074
Morag E. Ferguson, Larry D. Robertson, Brian V. Ford-Lloyd, H. John Newbury, Nigel Maxted, Contrasting genetic variation amongst lentil landraces from different geographical origins Euphytica. ,vol. 102, pp. 265- 273 ,(1998) , 10.1023/A:1018331432580
G. D. Duncan, Four new species and one new subspecies of Lachenalia (Hyacinthaceae) from arid areas of South Africa Bothalia. ,vol. 26, pp. 1- 9 ,(1996) , 10.4102/ABC.V26I1.682
K. Weising, H. Nybom, Markus Pfenninger, K. Wolff, Wieland Meyer, DNA Fingerprinting in Plants and Fungi ,(1995)
S.V. Irwin, P. Kaufusi, K. Banks, R. de la Peña, J.J. Cho, Molecular characterization of taro (Colocasia esculenta) using RAPD markers. Euphytica. ,vol. 99, pp. 183- 189 ,(1998) , 10.1023/A:1018309417762
John Welsh, Michael McClelland, Fingerprinting genomes using PCR with arbitrary primers Nucleic Acids Research. ,vol. 18, pp. 7213- 7218 ,(1990) , 10.1093/NAR/18.24.7213
Christie E. Williams, Dina A. St. Clair, Phenetic relationships and levels of variability detected by restriction fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and wild accessions of Lycopersicon esculentum. Genome. ,vol. 36, pp. 619- 630 ,(1993) , 10.1139/G93-083
Masatoshi Nei, Wen-Hsiung Li, Mathematical model for studying genetic variation in terms of restriction endonucleases Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 5269- 5273 ,(1979) , 10.1073/PNAS.76.10.5269