Long Noncoding RNA ZFPM2-AS1 Knockdown Restrains the Development of Retinoblastoma by Modulating the MicroRNA-515/HOXA1/Wnt/β-Catenin Axis

摘要: Purpose The tumor-initiating function of long non-coding RNA (lncRNA), zinc finger protein multitype 2 antisense 1 (ZFPM2-AS1) was reported in lung cancer, yet the relevance ZFPM2-AS1 retinoblastoma (RB), a malignancy representing 2.5% to 4% incidence cancers among children, has not been elucidated. Thus, we attempted assess effect and underlying mechanism RB progression. Methods First, comparing differentially expressed lncRNAs normal retinal tissues as well tissues, target lncRNA screened out. We then assayed expression three cell lines, carried out methylthiazol tetrazolium (MTT), transwell assays, flow cytometric analyses examine role si-ZFPM2-AS1 on behaviors. Following online database predication, correlations between microR-515 (miR-515) or homeobox A1 (HOXA1) were corroborated by dual-luciferase reporter gene assays. Quantitative real-time PCR along with Western blot assays fulfilled ascertain relevant genes. Results significantly overexpressed silencing curtailed growth metastasis cells both vitro vivo. Bioinformatic websites disclosed that might perform competing endogenous for miR-515 positively correlate HOXA1 activate Wnt/β-catenin signaling pathway. Conclusion Altogether, these data demonstrated interacted promote development via mediating miR-515, establishing promising therapeutic biomarker prognosis.