High-pressure liquid chromatographic analysis of anaerobic photoproducts of bilirubin-IX alpha in vitro and its comparison with photoproducts in vivo.

摘要: Abstract To carry out photochemical experiments under conditions similar to those prevailing for neonatal bilirubin metabolism in jaundice phototherapy, we have studied photoproducts produced by the action of light on a bilirubin--albumin solution and further clarified relationship between obtained from vitro vivo. (1) An accurate sensitive separation method high-pressure liquid chromatography anaerobic irradiation visible is described. (2) There were two main both vivo vitro. (3) Exact correspondence retention time chromatography, diazo-reactivity, thermal reversion absorption-spectrum maxima was observed unknown pigment photobilirubin-IX alpha biological fluids, comparable peaks 2 3 (4) The behaviour various solutions absence O2 (5) A lower affinity photoproducts, especially pigment, human serum albumin than with bilirubin-IX demonstrated gel-filtration method.