Dynamic assessment of human sperm DNA damage II: the effect of sperm concentration adjustment during processing

摘要: To evaluate the effect of sperm concentration adjustment in human ejaculates on DNA quality and longevity. Semen samples were obtained from 30 donors with a normal spermiogram. Following centrifugation, pellet was resuspended PBS, adjusted to 200, 100, 50, 25, 12, 6 × 106/mL. Each set incubated at 37 °C for 24 h, damage assessed using chromatin-dispersion test following 0 h, 2 h, 6 h, 24 h incubation. Sperm fragmentation (SDF) did not differ between selected experimental conditions T0; however, Kaplan–Meier estimates survival showed significant differences respect dilution time (all P values smaller than .001). semen 200 × 106/mL approximately 3.3 times higher when compared containing 25 × 106/mL 3.9 comparison 12 × 106/mL 2 h vitro Although there evidence individual variation SDF during incubation period, general finding that lower concentrations resulted slower rate fragmentation. Incubation spermatozoa ART purposes should be done below order avoid susceptibility molecule towards