In vitro selection of an allosteric ribozyme that transduces analytes to amplicons

摘要: We have selected an allosteric ribozyme ligase from a random sequence population that is activated up to 10,000-fold by oligonucleotide effectors. The conforms classic two-state model for allostery in which the equilibrium between inactive and active conformers dramatically altered presence of effector ligands. In generates templates can subsequently be amplified using conventional amplification technologies, such as RT-PCR. Thus, transduce (or convert) analytes into amplicons. demonstrate two potential diagnostic applications ligase: 'counting' short effectors RT-PCR, counting non-nucleic acid effector, ATP, ligation.