Method for producing amniotic mesenchymal stromal cell composition, method for cryopreserving the same, and therapeutic agent
作者： Akihiko Taguchi , Akira Kobayashi , Kenichi Yamahara , Shunsuke Ohnishi , Toshihiro Soma
DOI:
关键词: Inflammatory bowel disease 、 Mesenchymal stem cell 、 Thermolysin 、 Stromal cell 、 Collagenase 、 Dispase 、 Cancer research 、 Cryopreservation 、 Amnion 、 Chemistry
摘要: An object of the present invention is to provide a method for producing mesenchymal stromal cell composition, comprising conveniently and aseptically separating high-purity amnion-derived MSCs by performing enzyme treatment only once. According invention, following are provided: comprising: an amnion with collagenase thermolysin and/or dispase; filtering enzyme-treated through mesh; cryopreserved composition; therapeutic agent as active ingredient composition disease selected from graft-versus-host disease, inflammatory bowel systemic lupus erythematosus, liver cirrhosis, or radiation enteritis.
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