Luciferase-induced photoreductive uncaging of small-molecule effectors
作者： Eric Lindberg , Simona Angerani , Marcello Anzola , Nicolas Winssinger
DOI: 10.1038/S41467-018-05916-9
关键词: Ruthenium 、 Bioorthogonal chemistry 、 Effector 、 Analyte 、 Luciferase 、 Biophysics 、 Bioluminescence 、 Duocarmycin 、 Small molecule 、 Chemistry 、 General Biochemistry, Genetics and Molecular Biology 、 General Physics and Astronomy 、 General chemistry
摘要: Bioluminescence resonance energy transfer (BRET) is extensively used to study dynamic systems and has been utilized in sensors for studying protein proximity, metabolites, drug concentrations. Herein, we demonstrate that BRET can activate a ruthenium-based photocatalyst which performs bioorthogonal reactions. from luciferase the ruthenium uncage effector molecules with up 64 turnovers of catalyst, achieving concentrations >0.6 μM 10 nM construct. Using sensor, further catalysis be modulated response an analyte, analogous allosterically controlled enzymes. The BRET-induced reaction small-molecule drugs (ibrutinib duocarmycin) at biologically effective cellulo.
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