Mouse kidney cytidine deaminase. Purification and properties.
作者： R Tomchick , L D Saslaw , V S Waravdekar
DOI: 10.1016/S0021-9258(18)93407-1
关键词: Chromatography 、 Nucleoside 、 Fractionation 、 Dialysis 、 Aminohydrolase 、 Deoxycytidine 、 Denaturation (biochemistry) 、 Cytidine 、 Cytidine deaminase 、 Chemistry 、 Biochemistry
摘要: Abstract A nucleoside aminohydrolase was prepared in 60-fold relative purity from the nonparticulate fraction of mouse kidney by denaturation at 60°, fractionation with (NH4)2SO4, treatment alumina gel Cγ (pH 7.5), and dialysis 0.02 m acetate buffer 4.5). The optimal pH 6.5. Cytidine, deoxycytidine, 1-β-d-arabinofuranosylcytosine were deaminated, respective Km values 7.53 x 10-5, 7.25 1.01 10-3. Divalent metals not required for activity. Inhibition p-chloromercuribenzoate indicated participation sulfhydryl groups.
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