Cadaver lungs for transplantation. Effect of ventilation with alveolar gas.

摘要: In an effort to increase the donor pool for lung transplantation (LTX), we have demonstrated feasibility of LTX from circulation-arrested cadavers in a canine model. We hypothesized that ventilation cadaver with alveolar gas (20% O 2 , 5% CO balance N ) (AG) would be superior 100% oxygen (O after circulatory arrest donor. Twelve mongrel dogs were intubated, heparinized and euthanized by pentothal injection ventilated AG (n=6) or (n=6). Four hours later, animals underwent sternotomy, lungs flushed cold modified Euro-Collins solution, harvested, stored inflated iced slush. Left allotransplantation was performed, recipients made dependent on transplanted occlusion contralateral bronchus pulmonary artery. Recipient FiO 0.4 followed 8 hr. Total ischemic time 7.9 hr both groups. Pulmonary edema developed all ; one recipient survived 8-hr observation period poor oxygenation. contrast, three six -ventilated excellent exchange. Specimens before transplant evaluated histologically utilizing trypan blue exclusion as indicator cell viability. At organ retrieval 4 death, 6% cells nonviable lungs, compared 11% AG-ventilated lungs. Circulation-arrested prior function AG. Ventilation induces injury this Retrieval has potential increasing pool.