Detection of the rat adipose cell glucose transporter with antibody against the human red cell glucose transporter
作者： Thomas J. Wheeler , Ian A. Simpson , David C. Sogin , Peter C. Hinkle , Samuel W. Cushman
DOI: 10.1016/S0006-291X(82)80014-4
关键词: Microsome 、 Membrane protein 、 Cytochalasin B 、 Antibody 、 Insulin 、 Adipose tissue 、 Membrane 、 Glucose transporter 、 Biology 、 Biochemistry 、 Biophysics 、 Cell biology 、 Molecular biology
摘要: [ 125 1]-protein A and an affinity purified rabbit antibody against the human erythrocyte glucose transporter have been used to label rat adipose cell plasma low-density microsomal membrane proteins after transfer from SDS-polyacrylamide gels nitrocellulose paper. major labeled band is observed in both preparations with apparent molecular weight (approximately 45,000) similar that of transporter. When membranes are prepared cells incubated absence insulin, intensity this roughly 2-fold greater than membranes. Incubation a maximally stimulating concentration however, increases concomitantly decreases In cases, parallels transport systems determined by specific D-glucose-inhibitable 3 H] cytochalasin B binding. These results indicate immunologically
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