Human pancreas preservation prior to islet isolation. Cold ischemic tolerance.

摘要: We have retrospectively evaluated islet isolation records collected from 230 consecutive adult pancreases with 146 fulfilling all criteria for our evaluation. Fifty-six procured by local organ procurement team (33 before in situ vascular flush and 23 after University of Wisconsin [UW] solution) were compared 90 received distant centers that shipped UW solution flushing. Cold storage 3-26 hr preceded using collagenase digestion Ficoll purification. Recoveries islets assessed duplicate counts dithizone-stained aliquots Isolations considered successful if > 100,000 viable (islet equivalents to 150 microns) recovered Islet function was vitro glucose-stimulated perifusion the stimulation index (glucose stimulated over basal insulin secretion) 2. Eighty-three percent isolations locally procured, UW-flushed successful, as 86% stored 3 8 hr, 73% 16 storage, 38% following cold storage. Increasing duration prior associated an increased proportion failed decreased measures viability. The actual numbers per gram pancreas purification significantly reduced hypothermic hr. In viability isolated during showed a higher percentage shorter durations For isolation, reduced. conclude that, current methods available recover store cadaver donor currently used isolate human islets, yields purified decline been subjected 16-18