Primordial germ cell development in cultures of dispersed central disks of stage X chick blastoderms
作者： Malka Ginsburg , Hefzibah Eyal-Giladi
关键词: Histology 、 Incubation 、 Biology 、 Anatomy 、 Cellular differentiation 、 Cell–cell interaction 、 Blastoderm 、 Embryo 、 Cell culture 、 Cell biology 、 Somatic cell
摘要: Central disk fragments cut from stage X chick blastoderms were dispersed and cultured on glass coverslips. After 48 hr of incubation the cultures showed various degrees organization into three-layered aggregates in which no axis development was observed. Primordial germ cells (PGCs) detected ail cultures. The number PGCs found to be correlated initial cell concentration suspension. By regression analysis it that initiated 10 central disks or more, mean per fragment constant matched for intact control incubated same length time. It appears X, morphologic expression is related level differentiation somatic culture, which, turn, dependent culture.
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