Transfection of Malaria Parasites
作者： A.P. Waters , A.W. Thomas , M.R. van Dijk , C.J. Janse
关键词: Plasmodium berghei 、 Human parasite 、 Transgene 、 Malaria 、 Plasmodium falciparum 、 Plasmodium 、 Genetics 、 Selectable marker 、 Plasmodium knowlesi 、 Biology 、 General Biochemistry, Genetics and Molecular Biology 、 Molecular biology
摘要: The stable genetic transformation of three phylogenetically diverse species Plasmodium, the parasitic etiological agent malaria, is now possible. parasite haploid throughout vast majority its life cycle. Therefore with single selectable marker activity and protocols currently available, it possible not only to express introduced transgenes but also study effects site-specific homologous recombination such as gene knockout. Transgene expression will allow detailed many aspects cellular biology malaria parasites, for example, mechanisms underlying drug resistance protein trafficking. We describe here methods propagation two animal models (Plasmodium berghei Plasmodium knowlesi) transfection these human parasite, falciparum. Examples transgene are given.
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