Reduction of MDHAR activity in cherry tomato suppresses growth and yield and MDHAR activity is correlated with sugar levels under high light.
作者： Vincent Truffault , Noé Gest , Cécile Garchery , Alexandra Florian , Alisdair R. Fernie
DOI: 10.1111/PCE.12663
关键词: Cherry tomato 、 Dehydroascorbic acid 、 Horticulture 、 Ascorbic acid 、 Reductase 、 Peroxidase 、 Sucrose 、 Biochemistry 、 Photosynthesis 、 Sugar 、 Chemistry
摘要: Ascorbate is oxidized into the radical monodehydroascorbate (MDHA) through ascorbate oxidase or peroxidase activity non-enzymatically by reactive oxygen species. Regeneration of from MDHA ensured enzyme reductase (MDHAR). Previous work has shown that growth processes and yield can be altered modifying enzymes recycle ascorbate; therefore, we have studied similar in cherry tomato (Solanum lycopersium L.) under- overexpressing MDHAR. Physiological metabolic characterization these lines was carried out under different light conditions manipulating source-sink ratio. Independently regime, slower early all organs observed MDHAR silenced lines, decreasing final fruit yield. Photosynthesis as accumulation hexoses sucrose a light-dependent manner plantlets. Sucrose also repressed young fruits showed stronger decrease carbon limitation, phenotype partially restored reducing load. appear to involved control sugar metabolism associated could potential targets for improvement.
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