Protection of surface layer protein from Enterococcus faecium WEFA23 against Listeria monocytogenes CMCC54007 infection by modulating intestinal permeability and immunity.

摘要: Enterococcus faecium WEFA23 was previously found effectively against adherence and colonization of Listeria monocytogenes CMCC54007, which might be closely related to its surface layer protein (SLP). In this study, the protective SLP E. infection L. CMCC54007 systemically investigated. vitro assay showed that actively inhibited internalization into Caco-2 cell line, with decreasing mRNA level pro-inflammation cytokines virulence factors restoring destroyed intestinal barrier. vivo through excluding by 5 M LiCl represented increased body weight, reduced mortality counts in tissues mice. Further researches protected modulation permeability immunity, namely, it decreased fluorescein isothiocyanate (FITC)-Dextran serum, ameliorated colon structure, number goblet cells TJ (Claudin-1, Occludin, ZO-1) colon. For CD4+ CD8+ T liver, level, content pro-inflammatory IL-6, IL-1β, IFN-γ ,TNF-α, NO, restored structure liver spleen. Key Points•SLP colonization•SLP host barrier dysfunction•SLP cells.