Hypoxia-inducible factor 1 alpha contributes to cardiac healing in mesenchymal stem cells-mediated cardiac repair.
作者： Rubén Carrero , César Trigueros , Akaitz Dorronsoro , Jose María Sanchez-Puelles , Antonio Diez-Juan
关键词: Wound healing 、 Hypoxia-Inducible Factor 1-Alpha 、 Cancer research 、 Hypoxia (medical) 、 Cell adhesion 、 Downregulation and upregulation 、 Angiogenesis 、 Mesenchymal stem cell 、 Paracrine signalling 、 Biology 、 Immunology
摘要: Mesenchymal stem cells (MSC) are effective in treating myocardial infarction (MI) and previous reports demonstrated that hypoxia improves MSC self-renewal therapeutics. Considering hypoxia-inducible factor-1 alpha (HIF-1α) is a master regulator of the adaptative response to hypoxia, we hypothesized HIF-1α overexpression could mimic some mechanisms triggered by increase their therapeutic potential without stimulation. Transduction with lentivirus vectors (MSC-HIF) resulted increased cell adhesion migration, activation target genes coding for paracrine factors. When MSC-HIF were intramyocardially injected infarcted nude rats, significant improvement was found (after treatment rats MSC-HIF) terms cardiac function, angiogenesis, cardiomyocyte proliferation, reduction fibrotic tissue no induction hypertrophy. This finding provides evidences crucial role on biology suggests stabilization as novel strategy cellular therapies.
sci-hub.se 参考文章(56)
GL Wang, GL Semenza, Desferrioxamine induces erythropoietin gene expression and hypoxia-inducible factor 1 DNA-binding activity: implications for models of hypoxia signal transduction. Blood. ,vol. 82, pp. 3610- 3615 ,(1993) , 10.1182/BLOOD.V82.12.3610.3610
Duohong Zou, Zhiyuan Zhang, Dongxia Ye, Aifa Tang, Lianfu Deng, Wei Han, Jun Zhao, Shuhong Wang, Wenjie Zhang, Chao Zhu, Jian Zhou, Jiacai He, Yuanyin Wang, Feng Xu, Yuanliang Huang, Xinquan Jiang, Repair of critical-sized rat calvarial defects using genetically engineered bone marrow-derived mesenchymal stem cells overexpressing hypoxia-inducible factor-1α. Stem Cells. ,vol. 29, pp. 1380- 1390 ,(2011) , 10.1002/STEM.693
Raffi Bekeredjian, Chad B. Walton, Keith A. MacCannell, Jennifer Ecker, Fred Kruse, Joel T. Outten, David Sutcliffe, Robert D. Gerard, Richard K. Bruick, Ralph V. Shohet, Conditional HIF-1α Expression Produces a Reversible Cardiomyopathy PLoS ONE. ,vol. 5, pp. e11693- ,(2010) , 10.1371/JOURNAL.PONE.0011693
Gary R. Grotendorst, Connective tissue growth factor: a mediator of TGF-β action on fibroblasts Cytokine & Growth Factor Reviews. ,vol. 8, pp. 171- 179 ,(1997) , 10.1016/S1359-6101(97)00010-5
Suna Wang, Yifu Zhou, Oleg Andreyev, Robert F. Hoyt, Avneesh Singh, Timothy Hunt, Keith A. Horvath, Overexpression of FABP3 inhibits human bone marrow derived mesenchymal stem cell proliferation but enhances their survival in hypoxia Experimental Cell Research. ,vol. 323, pp. 56- 65 ,(2014) , 10.1016/J.YEXCR.2014.02.015
Fenxi Zhang, Junfang Wu, Ming Lu, Huaibin Wang, Huigen Feng, 5-Azacytidine inhibits proliferation and induces apoptosis of mouse bone marrow-derived mesenchymal stem cells Toxin Reviews. ,vol. 32, pp. 55- 59 ,(2013) , 10.3109/15569543.2013.846382
Daniel J Ceradini, Anita R Kulkarni, Matthew J Callaghan, Oren M Tepper, Nicholas Bastidas, Mark E Kleinman, Jennifer M Capla, Robert D Galiano, Jamie P Levine, Geoffrey C Gurtner, Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1 Nature Medicine. ,vol. 10, pp. 858- 864 ,(2004) , 10.1038/NM1075
Xian-Liang Tang, Gregg Rokosh, Santosh K. Sanganalmath, Fangping Yuan, Hiroshi Sato, Jianyao Mu, Shujing Dai, Chengxin Li, Ning Chen, Yong Peng, Buddhadeb Dawn, Greg Hunt, Annarosa Leri, Jan Kajstura, Sumit Tiwari, Gregg Shirk, Piero Anversa, Roberto Bolli, Intracoronary Administration of Cardiac Progenitor Cells Alleviates Left Ventricular Dysfunction in Rats With a 30-Day-Old Infarction Circulation. ,vol. 121, pp. 293- 305 ,(2010) , 10.1161/CIRCULATIONAHA.109.871905
Shan Ping Yu, Zheng Wei, Ling Wei, Preconditioning Strategy in Stem Cell Transplantation Therapy Translational Stroke Research. ,vol. 4, pp. 76- 88 ,(2013) , 10.1007/S12975-012-0251-0
Anna Grazia Recchia, Ernestina Marianna De Francesco, Adele Vivacqua, Diego Sisci, Maria Luisa Panno, Sebastiano Andò, Marcello Maggiolini, The G Protein-coupled Receptor 30 Is Up-regulated by Hypoxia-inducible Factor-1α (HIF-1α) in Breast Cancer Cells and Cardiomyocytes Journal of Biological Chemistry. ,vol. 286, pp. 10773- 10782 ,(2011) , 10.1074/JBC.M110.172247