Use of Transformed Roots for Root Development and Metabolism Studies and Progress in Characterizing Root-Specific Gene Expression

摘要: Following reports in the early 1980s that adventitious roots were transformed with Agrobacterium rhizogenes often grow rapidly hormone-free medium vitro, there has been an upsurge use of root cultures as a system to study metabolism and associated biology. As DNA transfer is involved interaction between A. higher plants, also great deal interest using this bacterium vector genetically manipulate plants. More than 460 plant species from over 100 families have by (Porter, 1991) many cases axenic reported vitro (Hamill Rhodes, 1992). In general, such are quite stable at gross chromosomal level, so long integrity meristem not disturbed, e.g., addition phytohormones (Aird et al., 1988a, b). Transformed used for secondary metabolite studies also, lesser extent, examine interactions soil organisms plants The recovery growth demonstrates containing Ri T-DNA can provide nutrient water uptake requirements, though few critically evaluated differences normal respect these functions.