Polyclonal activation of immunoglobulin secretion in B lymphocytes induced by staphylococcal peptidoglycan.

摘要: Staphylococcal peptidoglycan (PG) is a B cell mitogen and immunomodulator in mice. The ability of PG to induce the secretion polyclonal antibodies murine lymphocyte cultures was studied using protein A hemolytic plaque assay. as effective or more than lipopolysaccharide an inducer spleen, lymph node, bone marrow lymphocytes. highest numbers immunoglobulin (Ig) secreting cells were induced lowest cultures. All major classes Ig (IgM, IgG, IgA) induced, maximal Ig-secreting detected between days 4 5 culture. induction by Pg T cell, 2-mercaptoethanol, large extent macrophage independent.