A rapid method for screening inhibitor effects: Determination of I50 and its standard deviation

摘要: A simple method has been developed for the rapid screening of potential effectors on enzymatic reactions. Use is made linear relationship between inhibitor concentration I and corresponding value I(I − P), where P fraction inhibition given by I. This mode representation permits evaluation half-inhibitory doses (I50) calculation their standard deviations SI50. Theoretically, these parameters can be determined from results measurements carried out at a single concentration. The proved useful when applied to study number adrenal cortex cyclic AMP phosphodiesterase activity. model system was used assess advantages limitations procedure. approach should dealing with usual types enzyme mechanisms may interest in other biochemical fields, such as hormone-receptor interaction, provided that biological statistical are not overlooked.