Optimization of biolistic method for transient gene expression and production of agronomically useful transgenic Basmati rice plants.

摘要: We have developed a reproducible biolistic procedure for the efficient transformation of embryogenic suspension cells an improved aromatic Indica rice variety, Pusa Basmati 1. The β-glucuronidase gene was used to assay transient transformation; other plasmids carrying either potato protease inhibitor 2 (Pin2) gene, or late embryogenesis-abundant protein (LEA3) from barley, were optimization process and transgenic plant production. After procedure, over 600 transformants at least five fertile plants showing integrative obtained per bombarded filter. At 30% derived independent events. new involves use reporter useful genes driven by strong actin 1 (Act1) promoter, osmotic pre-conditioning 24 h on medium supplemented with 0.25 M mannitol prior bombardment, gold particles DNA delivery, regeneration high (1.0%) agarose concentration.