Quantitative assessments of cerebral vascular damage with a silicon rubber casting method in photochemically-induced thrombotic stroke rat models

摘要: Abstract Previous studies have described microvascular disturbances downstream of occluded large vessels arising during the acute phase (several hours) following cerebral ischemic insult. Prolonged may cause delayed neuronal cell death in penumbral regions, leading to expanded brain infarctions and poor neurological functional outcomes. The lack simple quantitative methods for investigating this microcirculation failure suggests need develop a new method clarifying precise distribution persistence post-ischemic disturbances. present study used silicone rubber casting analyses conditions photochemically-induced thromboembolic (PIT) stroke rat models. After procedure rats with PIT stroke, 6 μm-thick coronal section was obtained, density measurements infarct area serial were performed. major findings are as follows: (1) Silicone techniques can be applied microvessels same individual whom volume measured; (2) spatial assessed at core, penumbra, non-ischemic regions strongly suggest that affect expansion; (3) current demonstrated protective effects MK-801 on microvessels, indicating technique useful factors provide vascular protection. experimental introduced here would facilitate future evaluations agents.